U of T Technology & Startup Explorer

A novel method for sequencing polypeptides at the single-molecule level. This approach involves the use of a set of optical probes comprising variants of CIpS proteins from E. coli each capable of selectively binding a specific N-terminal amino acid.

Molecular imaging–based methods, assays, and reagents that are useful for sequencing proteins.

A cost-effective method for producing chitobiose using genetically modified bacteria. Researchers have used Streptomyces venezuelae, which possesses several secretable chitinolytic enzymes, to extracellularly convert chitin to chitobiose. Key to the invention is the knockout of chitobiose transporter proteins, which severely impairs the ability of the bacterium to import the chitobiose into the cell, leaving the metabolite available for harvesting from the highly enriched culture media.

Serum-free, T-cell media of comparable expansion potential to serum-based formulations. Using an AI-based, media-optimization algorithm (HiDiNeu) they optimized 14 factors to yield numerous high-performing media formulations.